Vascular smooth muscle contraction；Chemokine signaling pathway；Proteoglycans in cancer； Wnt signaling pathway；TGF-beta signaling pathway；Axon guidance；Focal adhesion；Leukocyte transendothelial migration；Regulation of actin cytoskeleton；Pathogenic Escherichia coli infection；Shigellosis；Salmonella infection

The subsequent luciferase assay verified TUG1 was a target of miR-335-5p. Furthermore, the results of a real-time quantitative PCR showed that TUG1 and miR-335-5p could affect each other's expression. respectively. Finally, we affirmed that TUG1 affected ROCK1 expression and ROCK1-mediated migration/invasion by working as a competitive endogenous RNA (ceRNA) via miR-335-5p.

Long non-coding RNA TUG1 promotes migration and invasion by acting as a ceRNA of miR-335-5p in osteosarcoma cells