Regulation of autophagy

In conclusion, PVT1 overexpression increased the expression of Atg7 and Beclin1 by targeting miR-186, which induced protective autophagy, thus promoting glioma vascular endothelial cell proliferation, migration, and angiogenesis.

PVT1 affects growth of glioma microvascular endothelial cells by negatively regulating miR-186

HIF-1 signaling pathway；NF-kappa B signaling pathway；Protein processing in endoplasmic reticulum；Pathways in cancer；Small cell lung cancer；Prostate cancer；PI3K-Akt signaling pathway；Apoptosis；Focal adhesion； Neurotrophin signaling pathway；Cholinergic synapse；Amyotrophic lateral sclerosis (ALS)；Colorectal cancer；Hepatitis B；Epstein-Barr virus infection； Toxoplasmosis；Tuberculosis

Moreover, silencing PVT1 by siRNA inhibited BCL2, CCND1, and FASN protein expression via miR-195 in osteosarcoma cells, and BCL2 inhibited the si-PVT1#1-induced apoptosis of U2OS cells.

Long non-coding RNA PVT1 promotes osteosarcoma development by acting as a molecular sponge to regulate miR-195

Regulation of autophagy

In conclusion, PVT1 overexpression increased the expression of Atg7 and Beclin1 by targeting miR-186, which induced protective autophagy, thus promoting glioma vascular endothelial cell proliferation, migration, and angiogenesis.

PVT1 affects growth of glioma microvascular endothelial cells by negatively regulating miR-186

Cell cycle；p53 signaling pathway；Endometrial cancer；Melanoma；Thyroid cancer；PI3K-Akt signaling pathway；Pancreatic cancer；Wnt signaling pathway；Hippo signaling pathway；Prostate cancer；Acute myeloid leukemia；Focal adhesion；Viral myocarditis；Viral carcinogenesis；Colorectal cancer；Jak-STAT signaling pathway；Hepatitis B；Measles；Proteoglycans in cancer；Bladder cancer；Non-small cell lung cancer；HTLV-I infection；Small cell lung cancer；Pathways in cancer；Glioma；Chronic myeloid leukemia

Moreover, silencing PVT1 by siRNA inhibited BCL2, CCND1, and FASN protein expression via miR-195 in osteosarcoma cells, and BCL2 inhibited the si-PVT1#1-induced apoptosis of U2OS cells.

Long non-coding RNA PVT1 promotes osteosarcoma development by acting as a molecular sponge to regulate miR-195

Moreover, PVT1 might inhibit the expression of miR-152 and increased the expression of CD151 and FGF2 through regulating miR-152. PVT1 was positively associated with CD151 and FGF2 expression in GC tissues.

Long Noncoding RNA PVT1 Acts as a "Sponge" to Inhibit microRNA-152 in Gastric Cancer Cells

Moreover, PVT1 might inhibit the expression of miR-152 and increased the expression of CD151 and FGF2 through regulating miR-152.

Long Noncoding RNA PVT1 Acts as a "Sponge" to Inhibit microRNA-152 in Gastric Cancer Cells.

PVT1 directly interacts with EZH2 and the complex anchors in the promoter region of miR-195.

LncRNA PVT1 epigenetically silences miR-195 and modulates EMT and chemoresistance in cervical cancer cells

Mechanistically, we verified that PVT1 binds to EZH2, recruits EZH2 to the miR-200b promoter, increases histone H3K27 trimethylation level on the miR-200b promoter, and inhibits miR-200b expression.

Long noncoding RNA PVT1 promotes cervical cancer progression through epigenetically silencing miR-200b

Fatty acid biosynthesis；Metabolic pathways； Insulin signaling pathway

Moreover, silencing PVT1 by siRNA inhibited BCL2, CCND1, and FASN protein expression via miR-195 in osteosarcoma cells, and BCL2 inhibited the si-PVT1#1-induced apoptosis of U2OS cells.

Long non-coding RNA PVT1 promotes osteosarcoma development by acting as a molecular sponge to regulate miR-195

MAPK signaling pathway；PI3K-Akt signaling pathway；Regulation of actin cytoskeleton；Pathways in cancer；Proteoglycans in cancer；Melanoma

Moreover, PVT1 might inhibit the expression of miR-152 and increased the expression of CD151 and FGF2 through regulating miR-152. PVT1 was positively associated with CD151 and FGF2 expression in GC tissues.

Long Noncoding RNA PVT1 Acts as a "Sponge" to Inhibit microRNA-152 in Gastric Cancer Cells

MAPK signaling pathway；PI3K-Akt signaling pathway；Regulation of actin cytoskeleton； Pathways in cancer；Proteoglycans in cancer；Melanoma

Moreover, PVT1 might inhibit the expression of miR-152 and increased the expression of CD151 and FGF2 through regulating miR-152.

Long Noncoding RNA PVT1 Acts as a "Sponge" to Inhibit microRNA-152 in Gastric Cancer Cells.

HIF-1 signaling pathway；mTOR signaling pathway；Pathways in cancer；Proteoglycans in cancer；Renal cell carcinoma

In addition, PVT1 could directly interact with miR-186 in GC cells and this interaction lead to the inhibition of downstream of HIF-1α expression.

The long noncoding RNA PVT1 functions as a competing endogenous RNA by sponging miR-186 in gastric cancer

Starch and sucrose metabolism；Glycolysis / Gluconeogenesis；Fructose and mannose metabolism；Galactose metabolism；Amino sugar and nucleotide sugar metabolism；Butirosin and neomycin biosynthesis；Metabolic pathways；HIF-1 signaling pathway；Insulin signaling pathway；Type II diabetes mellitus ；Carbohydrate digestion and absorption

Functionally, knockdown of PVT1 exerted tumor-suppressive effect by suppressing cell proliferation, cell cycle progression, and invasion in vitro, whereas miR-497 inhibitor partially abolished the inhibition effect of si-PVT1. Overexpression of HK2 attenuated the miR-497 induced inhibition of cell growth and motility. Taken together, these findings suggested that PVT1 contributes to OS cell glucose metabolism, cell proliferation, and motility through the miR-497/HK2 pathway, and revealed a novel relation between lncRNA and the alteration of glycolysis in OS cells.

Long non-coding RNA PVT1 promotes glycolysis and tumor progression by regulating miR-497/HK2 axis in osteosarcoma.

Here, we showed that PVT1 expression is significantly up-regulated in ESCC tumor samples compared with their normal counterparts. Knockdown of PVT1 suppressed tumor growth in vitro and in vivo. Further studies revealed that silence of PVT1 lead to up-regulation of miR-203, and vice versa. Moreover, LASP1 was found to be downregulated after knockdown of PVT1 and overexpression of LASP1 attenuated the tumor-suppressive roles of PVT1 knockdown.

Upregulation of the long non-coding RNA PVT1 promotes esophageal squamous cell carcinoma progression by acting as a molecular sponge of miR-203 and LASP1.

Hedgehog signaling pathway；Pathways in cancer；Proteoglycans in cancer；Basal cell carcinoma

PTCH1 demethylation caused by miR-152 was responsible for the effects of PVT1 knockdown on PTCH1 expression.

Long non-coding RNA PVT1 activates hepatic stellate cells through competitively binding microRNA-152

Furthermore, bioinformatics analysis was utilized to show that PVT1 contains binding site for miR-448 and an inverse correlation between PVT1 and miR-448 was obtained in PC specimens. Additionally, dual luciferase reporter assay, RNA-binding protein immunoprecipitation (RIP) and applied biotin-avidin pulldown system were applied to further confirm that PVT1 directly bind with microRNA binding site harboring in the PVT1 sequence. Then, SERBP1 was identified as a target of miR-448 according to the gene expression array analysis of PC clinical samples

LncRNA-PVT1 promotes pancreatic cancer cells proliferation and migration through acting as a molecular sponge to regulate miR-448.

Proteoglycans in cancer

We then confirmed that PVT1 acts as a sponge for miRNA-186-5p and positively regulates Twist1 by a sponge effect. Therefore, this study has revealed a novel MECHANISM for the promotion of EMT in prostate cancer by PVT1. Our findings suggest that the PVT1/miR-186/Twist1 regulatory axis may be a new therapeutic target for prostate cancer.

Long noncoding RNA PVT1 promotes EMT via mediating microRNA-186 targeting of Twist1 in prostate cancer