Dopaminergic synapse；ErbB signaling pathway；MAPK signaling pathway；Prostate cancer；Pathways in cancer；Melanoma；Pancreatic cancer；Chemokine signaling pathway；Fc epsilon RI signaling pathway；HIF-1 signaling pathway；Progesterone-mediated oocyte maturation；Colorectal cancer；HTLV-I infection；mTOR signaling pathway；Toll-like receptor signaling pathway；Toxoplasmosis；Epstein-Barr virus infection；Proteoglycans in cancer；Influenza A；Cholinergic synapse；Fc gamma R-mediated phagocytosis；Neurotrophin signaling pathway；Jak-STAT signaling pathway；T cell receptor signaling pathway；Acute myeloid leukemia；Glioma；Small cell lung cancer；Non-small cell lung cancer；PI3K-Akt signaling pathway； Apoptosis；VEGF signaling pathway；Hepatitis C；Carbohydrate digestion and absorption；Chagas disease (American trypanosomiasis)；Measles；Adipocytokine signaling pathway；Tuberculosis； Osteoclast differentiation；B cell receptor signaling pathway；Hepatitis B；Focal adhesion；Estrogen signaling pathway；Insulin signaling pathway；Tight junction；Endometrial cancer；Chronic myeloid leukemia；Renal cell carcinoma

In addition, overexpression of H19 in GBC-SD cells downregulated miR-194-5p and markedly increased AKT2 expression, and miR-194-5p mimic reversed these effects.

Long noncoding RNA H19 contributes to gallbladder cancer cell proliferation by modulated miR-194-5p targeting AKT2

We also show that Malat1 functions as a competing endogenous RNA (ceRNA) for miR-206. Because miR-206 directly suppresses expression of ANXA2 and KRAS, which are thought to promote GBC progression, Malat1 binding of miR-206 in GBC tissue and cells has an oncogenic effect. Conversely, Malat1 knockdown inhibits proliferation and invasion by GBC cells while increasing apoptosis.

Long non-coding RNA Malat1 promotes gallbladder cancer development by acting as a molecular sponge to regulate miR-206

Mechanistically, MINCR/miR-26a-5p/EZH2 axis was found to be involved in cell proliferation, cell invasive and apoptosis in GBC cells.

Long non-coding RNA MINCR promotes gallbladder cancer progression through stimulating EZH2 expression

QRT-PCR and Western-blotting assays demonstrated that H19 silencing down-regulated, whereas over-expression enhanced the expression of miR-342-3p targeting FOXM1 through competitively 'sponging' miR-342-3p.

Long non-coding RNA H19 regulates FOXM1 expression by competitively binding endogenous miR-342-3p in gallbladder cancer

Natural killer cell mediated cytotoxicity；MAPK signaling pathway；ErbB signaling pathway；Chemokine signaling pathway；PI3K-Akt signaling pathway；Insulin signaling pathway；Hepatitis C；Long-term potentiation；Progesterone-mediated oocyte maturation；Dorso-ventral axis formation；Axon guidance；Endometrial cancer；Regulation of actin cytoskeleton；VEGF signaling pathway；Long-term depression；Estrogen signaling pathway；Cholinergic synapse；Melanogenesis；Hepatitis B；Viral carcinogenesis；Tight junction；Neurotrophin signaling pathway；GnRH signaling pathway；Gap junction；Glioma；T cell receptor signaling pathway； Alcoholism；Prostate cancer；Serotonergic synapse；Bladder cancer；Non-small cell lung cancer；Chronic myeloid leukemia；HTLV-I infection；B cell receptor signaling pathway；Pathways in cancer；Aldosterone-regulated sodium reabsorption；Colorectal cancer；Melanoma；Acute myeloid leukemia；Proteoglycans in cancer；Pancreatic cancer；Renal cell carcinoma；Thyroid cancer；Fc epsilon RI signaling pathway

We also show that Malat1 functions as a competing endogenous RNA (ceRNA) for miR-206. Because miR-206 directly suppresses expression of ANXA2 and KRAS, which are thought to promote GBC progression, Malat1 binding of miR-206 in GBC tissue and cells has an oncogenic effect. Conversely, Malat1 knockdown inhibits proliferation and invasion by GBC cells while increasing apoptosis.

Long non-coding RNA Malat1 promotes gallbladder cancer development by acting as a molecular sponge to regulate miR-206

PI3K-Akt signaling pathway

Real-time quantitative PCR and Western blot analysis indicated that MALAT1 regulated Myeloid cell leukaemia-1 (MCL-1) expression as a competing endogenous RNA (ceRNA) for miR-363-3p in GBC cells.

The lncRNA MALAT1 functions as a competing endogenous RNA to regulate MCL-1 expression by sponging miR-363-3p in gallbladder cancer